Maninder Singh Setia
Department of Epidemiologist, MGM Institute of Health Sciences, Navi Mumbai, Maharashtra, India

Correspondence Address:
Dr. Maninder Singh Setia
MGM Institute of Health Sciences, Navi Mumbai, Maharashtra
India

Source of Support: None, Conflict of Interest: None

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DOI: 10.4103/0019-5154.182410

Abstract

Cross-sectional study design is a type of observational study design. In a cross-sectional study, the investigator measures the outcome and the exposures in the study participants at the same time. Unlike in case–control studies (participants selected based on the outcome status) or cohort studies (participants selected based on the exposure status), the participants in a cross-sectional study are just selected based on the inclusion and exclusion criteria set for the study. Once the participants have been selected for the study, the investigator follows the study to assess the exposure and the outcomes. Cross-sectional designs are used for population-based surveys and to assess the prevalence of diseases in clinic-based samples. These studies can usually be conducted relatively faster and are inexpensive. They may be conducted either before planning a cohort study or a baseline in a cohort study. These types of designs will give us information about the prevalence of outcomes or exposures; this information will be useful for designing the cohort study. However, since this is a 1-time measurement of exposure and outcome, it is difficult to derive causal relationships from cross-sectional analysis. We can estimate the prevalence of disease in cross-sectional studies. Furthermore, we will also be able to estimate the odds ratios to study the association between exposure and the outcomes in this design.

Keywords: Cross-sectional studies, design, limitations, strengths

Introduction

Design

Figure 1: Example of a cross-sectional study Click here to view

Examples of Cross-Sectional Studies

Antibiotic resistance in  Propionibacterium acnes  ana et al., 2016)

Measurements in a Cross-Sectional Study

1. Cross-sectional study designs may be used for population-based surveys
   
   Example: We are interested to know the prevalence of vitiligo in a village. We design a population-based survey to assess the prevalence of this condition. We go to all the houses that were supposed to be included in the study and examine the population. The total sample surveyed is 5686. Of these, we found that 98 individuals have vitiligo. Thus, the prevalence of vitiligo in this community is: Prevalence = 98/5686 or 17.23/1000 population
   
   
2. Cross-sectional studies may also be used for estimating the prevalence in clinic-based studies.Example: Research question – What is the prevalence of HIV in patients presenting with an STI?
   
   We evaluate 300 patients with an STI clinic. We record this history, clinical examination, and test them for HIV antibodies (using ELISA) during their first visit to the clinic. We find that 60 of these individuals are HIV infected. Thus, we have detected a prevalence of 20% HIV infection among our STI patients. This type of study will be classified as a cross-sectional study. Kindly note that this being a clinic-based study, it may have all the limitations of a clinic-based study. Thus, the prevalence from these data may have limited generalizability. Nonetheless, this type of study design will be classified as a cross-sectional study.
3. Cross-sectional studies may also be used to calculate the ORs
   
   For example, if we wish to understand the association between gender and HIV status, we will able to create a 2 × 2 table for the above-mentioned cross-sectional study. Of the 300 individuals evaluated, we have recruited 200 male and 100 female participants. Of the 60 HIV-infected individuals, 50 are males and 10 are females. The 2 × 2 table will be as follows:
   
   

   

   
   
   The OR (as discussed in the earlier methodology series – II case–control studies) is AD/BC or 50*90/10*150. Thus, the OR is 3.0. The interpretation of this OR is that males had a higher odds of being HIV infected compared with females. Since the OR is >1, the outcome is more likely in those exposed (males) compared with those who are not exposed (females). However, we will require confidence intervals to comment on further interpretation of the OR.

Strengths of a Cross-Sectional Study

1. Cross-sectional studies can usually be conducted relatively faster and are inexpensive – particularly when compared with cohort studies (prospective)
2. These are studies are conducted either before planning a cohort study or a baseline in a cohort study. These types of designs will give us information about the prevalence of outcomes or exposures; this information will be useful for designing the cohort study
3. These study designs may be useful for public health planning, monitoring, and evaluation. For example, sometimes the National AIDS Programme conducted cross-sectional sentinel surveys among high-risk groups and ante-natal mothers every year to monitor the prevalence of HIV in these groups.

Limitations of a Cross-Sectional Study

1. Since this is a 1-time measurement of exposure and outcome, it is difficult to derive causal relationships from cross-sectional analysis
2. These studies are also prone to certain biases. For example, we wish to study the relation between diet and exercise and being overweight/obese. We conduct a cross-sectional study and recruit 250 individuals. We assess their dietary habits, exercise habits, and body mass index at one point of time in a cross-sectional survey. However, individuals who are overweight/obese have started to exercise more or altered their feeding habits (eat more salads). Hence, in a cross-sectional survey, we may find that overweight/obese individuals are also more likely to eat salads and exercise more. Thus, we have to be careful about interpreting the associations and direction of associations from a cross-sectional survey
3. The prevalence of an outcome depends on the incidence of the disease as well as the length of survival following the outcome. For example, even if the incidence of HIV (number of new cases) goes down in one particular community, the prevalence (total number of cases – old as well as new) may increase. This may be due to cumulative HIV positive cases over a period. Thus, just performing cross-sectional surveys may not be sufficient to understand disease trends in this situation.

Additional Points

1. The National AIDS Control Organisation's Sentinel Surveillance of HIV is an example of “serial cross-sectional study” or “serial survey.” This may be less expensive compared with a cohort study
   
   Sentinel Surveillance in Antenatal Clinic: The surveillance recruits consecutive consenting pregnant women, aged 15–45 years in these clinics. The exercise has been in place for nearly two decades. The formal annual sentinel surveillance was instituted in 1998. The surveillance provided data on the prevalence of HIV infection in antenatal women, and thus, the trends of HIV infection in this population
   
   Such surveys are also conducted in female sex workers, men who have sex with men, and people who inject drugs, migrants, truckers, and male-to-female transgendered people. Repeated cross-sectional surveys provide useful information on the prevalence of HIV in these groups [Figure 2]. It can be seen that the prevalence has, in general, reduced over the past decade in these groups. Thus, repeated cross-sectional surveys are also useful to monitor the trends over a period.

   Figure 2: Data from National HIV Sentinel Surveillance (2003–2011)*. *Graph generated from data in the National HIV Sentinel Surveillance Click here to view

   

2. We will discuss the previous study by Sardana et al . They conducted one cross-sectional survey to assess the resistance patterns in P. acnes . If the authors conduct the same study consecutively for two more years, they will provide information on the changing resistance patterns in P. acnes. This will be an example of a serial cross-sectional study.

Summary

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