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LETTER TO EDITOR
Year : 2022  |  Volume : 67  |  Issue : 2  |  Page : 203
The use of buccal micronucleus assay for biomonitoring systemic lupus erythematosus: Is it possible?


From the Department of Biosciences, Federal University of Sao Paulo, UNIFESP, SP, Brazil

Date of Web Publication13-Jul-2022

Correspondence Address:
Daniel A Ribeiro
From the Department of Biosciences, Federal University of Sao Paulo, UNIFESP, SP
Brazil
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/ijd.IJD_764_20

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How to cite this article:
W. de Morais KD, Malacarne IT, Viana Md, Ribeiro DA. The use of buccal micronucleus assay for biomonitoring systemic lupus erythematosus: Is it possible?. Indian J Dermatol 2022;67:203

How to cite this URL:
W. de Morais KD, Malacarne IT, Viana Md, Ribeiro DA. The use of buccal micronucleus assay for biomonitoring systemic lupus erythematosus: Is it possible?. Indian J Dermatol [serial online] 2022 [cited 2022 Aug 17];67:203. Available from: https://www.e-ijd.org/text.asp?2022/67/2/203/350815




Sir,

We read the manuscript recently published by Assiri et al.[1] in the Indian Journal of Dermatology titled “Correlation of Buccal Micronucleus with Disease Activity Score Using Buccal Micronucleus Cytome Analysis (BMCA) in Systemic Lupus Erythematosus” with much interest. In this article, the authors failed to detect high frequencies of micronucleus in patients suffering from systemic lupus erythematosus. In addition, no correlations, such as micronucleus frequency and disease activity/duration were achieved. However, it is important to properly discuss the scientific approach for a correct understanding of the paper.

In Materials and Methods, it was stated “MN frequencies were determined from a total of 1,000 cells.” In the following paragraph, it was mentioned, “An average of 2,000 epithelial cells was observed for each individual.” In fact, how many cells were evaluated per individual? This needs clarification. Moreover, it was written, “Scoring criteria for the various distinct cell types and nuclear anomalies were evaluated in the BMCA, as described by Tolbert et al.[2]” Nevertheless, what cell types and nuclear anomalies other than micronucleus were evaluated in the study? It is important to emphasize that Tolbert et al.[2] have described several metanuclear changes indicative of cellular death (cytotoxicity) for the buccal micronucleus assay, such as pyknosis, karyorrhexis, and karyolysis. The approach is very interesting because cytotoxicity is potential for bias in the micronucleus assay. For example, if cytotoxicity is increased, then the micronucleus frequency decreased because micronucleated cells are lost as a result of cellular death. Certainly, the disease and/or therapy are able to induce cytotoxicity in mammalian cells.

Finally, although some correlations were performed, as for example, micronucleus and disease activity (P = 0.079) or duration of systemic lúpus erythematous (P = 0.934), where were these data presented in the manuscript? Certainly, this is very important to evaluate the paper with accuracy.

We believe that these comments are useful for a better understanding of the important article for biomonitoring patients with systemic lupus erythematous.

Acknowledgments

MBV and DAR are researchers on Productivity at CNPq (Conselho Nacional de Desenvolvimento Cientifico e Tecnologico).

Financial support and sponsorship

Nil.

Conflicts of interest

There are no conflicts of interest.



 
   References Top

1.
Assiri K, Hameed MS, Dawasaz AA, Alamoudi E, Asiri AM, Hitesh V, et al. Correlation of buccal micronucleus with disease activity score using buccal micronucleus cytome analysis (BMCA) in systemic lupus erythematosus. Indian J Dermatol 2020;65:265-8.  Back to cited text no. 1
  [Full text]  
2.
Tolbert PE, Shy CM, Allen JW. Micronuclei and other nuclear anoma-lies in buccal smears: Methods development. Mutation Res 1992;271:69-77.  Back to cited text no. 2
    




 

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