Indian Journal of Dermatology
ORIGINAL ARTICLE
Year
: 2021  |  Volume : 66  |  Issue : 4  |  Page : 331--336

The Difference in Expression of Autophagy-Related Proteins in Lesional and Perilesional Skin in Adult Patients with Active and Stable Generalized Vitiligo—A Cross-Sectional Pilot Study


Haiyan Yu1, Xiaoxia Lin1, Yaoyao Huang1, Hao Cheng1, Oliver Seifert2 
1 Department of Dermatology, Sir Run Run Shaw Hospital, Zhejiang University Medical College, 3 Qinchun Road East, Hangzhou, Zhejiang, China
2 Division of Dermatology and Venereology, Ryhov Hospital, Region Jönköping County, Jönköping; Department of Clinical and Experimental Medicine, Faculty of Medicine and Health Sciences, Linköping University, Linköping, Sweden

Correspondence Address:
Haiyan Yu
Department of Dermatology, Sir Run Run Shaw Hospital, Zhejiang University Medical College, Hangzhou, Zhejiang 310016
China

Background: Autophagy plays an important role in maintaining intracellular homeostasis and is essential for cell survival and cell death. Dysfunction of autophagy has been described in many autoimmune diseases but data on vitiligo are scarce. Aims: The aim of this pilot study was to investigate the expression of autophagy-related proteins in patients with vitiligo. Methods: Western blotting was used to analyze the expression of microtubule-associated protein light chain 3 (LC3II/I), autophagy-related gene 5 (Agt5), mammalian target of rapamycin (mTOR) and p62 in lesional and perilesional vitiligo skin from seven patients with active generalized vitiligo and nine patients with stable generalized vitiligo compared to control skin from six healthy subjects. Results: Our data showed increased expression of the autophagy marker LC3II/I and decreased p62 protein expression in lesional skin of active and stable vitiligo compared to control skin (P < 0.01). No significant difference in the expression of LC3II/I and p62 was found in perilesional skin of active vitiligo patients (P > 0.05) compared to control skin. Expression of LC3II/I in stable vitiligo lesional skin was higher and p62 expression was lower compared to active vitiligo lesional skin (P < 0.01). Decreased p62 expression was shown in perilesional skin of stable vitiligo patients (P < 0.05). Agt5 protein in lesional and perilesional skin of both active and stable vitiligo patients were increased (P < 0.01 and P< 0.05) compared to control skin. The expression of mTOR protein in lesional and perilesional skin of active and stable vitiligo patients was significantly lower than in control skin (P < 0.01). Conclusions: The present study indicates increased autophagy in lesional skin in vitiligo patients. Stable vitiligo lesional skin showed increased autophagy compared to active vitiligo lesional skin. Missing activation of autophagy in active vitiligo perilesional skin suggests disturbed autophagy to be associated with vitiligo.


How to cite this article:
Yu H, Lin X, Huang Y, Cheng H, Seifert O. The Difference in Expression of Autophagy-Related Proteins in Lesional and Perilesional Skin in Adult Patients with Active and Stable Generalized Vitiligo—A Cross-Sectional Pilot Study.Indian J Dermatol 2021;66:331-336


How to cite this URL:
Yu H, Lin X, Huang Y, Cheng H, Seifert O. The Difference in Expression of Autophagy-Related Proteins in Lesional and Perilesional Skin in Adult Patients with Active and Stable Generalized Vitiligo—A Cross-Sectional Pilot Study. Indian J Dermatol [serial online] 2021 [cited 2021 Dec 6 ];66:331-336
Available from: https://www.e-ijd.org/article.asp?issn=0019-5154;year=2021;volume=66;issue=4;spage=331;epage=336;aulast=Yu;type=0