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Figure 1: (A) Agarose gel electrophoresis showing the application of dermatophyte-specific PCR on dermatological specimens: Lane 1: Negative control, Lane 2: Nail clipping - Positive, Lane 3: Nail clipping - Positive, Lane 4: Nail clipping - Positive, Lane 5: Blister top - Positive, Lane 6: Blister top - Positive, Lane 7: Nail clipping- Positive, Lane 8: Nail clipping - Positive, Lane 9: Nail clipping - Positive, Lane 10: Nail clipping - Positive, Lane 11: Nail clipping - Positive, Lane 12: Nail clipping - Positive, Lane 13: Nail clipping - Positive, Lane 14: Nail clipping - Negative, Lane 15: Blister top - Positive, Lane 16: Nail clipping - Positive, Lane 17: Positive control: T. rubrum standard strain DNA, Lane 18: Mo; (B) Agarose gel electrophoresis showing the application of ITS PCR on dermatological specimens: Lane 1: Negative control, Lane 2: Nail clipping - Positive, Lane 3: Nail clipping - Positive, Lane 4: Nail clipping - Positive, Lane 5: Blister top - Positive, Lane 6: Blister top - Positive, Lane 7: Nail clipping - Positive, Lane 8: Nail clipping - Positive, Lane 9: Nail clipping - Positive, Lane 10: Nail clipping - Positive, Lane 11: Nail clipping - Positive, Lane 12: Nail clipping - Positive, Lane 13: Nail clipping- Positive, Lane 14: Nail clipping - Negative, Lane 15: Blister top - Positive, Lane 16: Nail clipping - Positive, Lane 17: Positive control: T. rubrum standard strain DNA, Lane 18: Molecular weight marker - Hae-III digest of Phi

Figure 1: (A) Agarose gel electrophoresis showing the application of dermatophyte-specific PCR on dermatological specimens: Lane 1: Negative control, Lane 2: Nail clipping - Positive, Lane 3: Nail clipping - Positive, Lane 4: Nail clipping - Positive, Lane 5: Blister top - Positive, Lane 6: Blister top - Positive, Lane 7: Nail clipping- Positive, Lane 8: Nail clipping - Positive, Lane 9: Nail clipping - Positive, Lane 10: Nail clipping - Positive, Lane 11: Nail clipping - Positive, Lane 12: Nail clipping - Positive, Lane 13: Nail clipping - Positive, Lane 14: Nail clipping - Negative, Lane 15: Blister top - Positive, Lane 16: Nail clipping - Positive, Lane 17: Positive control: T. rubrum standard strain DNA, Lane 18: Mo; (B) Agarose gel electrophoresis showing the application of ITS PCR on dermatological specimens: Lane 1: Negative control, Lane 2: Nail clipping - Positive, Lane 3: Nail clipping - Positive, Lane 4: Nail clipping - Positive, Lane 5: Blister top - Positive, Lane 6: Blister top - Positive, Lane 7: Nail clipping - Positive, Lane 8: Nail clipping - Positive, Lane 9: Nail clipping - Positive, Lane 10: Nail clipping - Positive, Lane 11: Nail clipping - Positive, Lane 12: Nail clipping - Positive, Lane 13: Nail clipping- Positive, Lane 14: Nail clipping - Negative, Lane 15: Blister top - Positive, Lane 16: Nail clipping - Positive, Lane 17: Positive control: T. rubrum standard strain DNA, Lane 18: Molecular weight marker - Hae-III digest of Phi