Figure 3: (A) Agarose gel electrophoresis of PCR-RFLP performed on ITS PCR amplicons to identify dermatophytes: Lane 1: Undigested product (595 bp), Lane 2: Digested product of M. gypseum (420, 95 bp), Lanes 3, 6, 7, 8, 9 and 10: Digested product of T. rubrum (300, 200, 95 bp), Lane 5: Digested product of E. floccosum (350, 95 bp), MW: Molecular weight marker – Hinf-I digest of Ö X bacteriophage DNA; (b) Agarose gel electrophoresis of PCR-RFLP performed on ITS PCR amplicons to identify nondermatophyte etiology: Lane 1: Undigested product C. albicans ATCC 24433 595 bp, Lane 2: Digested product identifi ed as A. niger (440, 150 bp), Lane 3: Digested product identifi ed as C. albicans (331, 150, 100 bp), Lane 4: Digested product identified as C. tropicalis (351, 150, 100, 50 bp), Lane 5: Digested product identifi ed as C. parapsilosis (351, 150, 100, 50 bp), Lane 6: Digested product identified as A. fumigatus (301, 160,150 bp), Lane 7: Digested product identifi ed as A. flavus (400, 195 bp), Lane 8: Digested product identified as A. fumigatus (301, 160, 150 bp), MW: Molecular weight marker– Ö X 174 bacteriophage DNA Hinf-I di